Investigation of lignin deposition on cellulose during hydrothermal pretreatment, its effect on cellulose hydrolysis, and underlying mechanisms.
Identifieur interne : 002156 ( Main/Exploration ); précédent : 002155; suivant : 002157Investigation of lignin deposition on cellulose during hydrothermal pretreatment, its effect on cellulose hydrolysis, and underlying mechanisms.
Auteurs : Hongjia Li [États-Unis] ; Yunqiao Pu ; Rajeev Kumar ; Arthur J. Ragauskas ; Charles E. WymanSource :
- Biotechnology and bioengineering [ 1097-0290 ] ; 2014.
Descripteurs français
- KwdFr :
- Bois (MeSH), Cellulases (métabolisme), Cellulose (composition chimique), Cellulose (effets des radiations), Cellulose (métabolisme), Cellulose (ultrastructure), Hydrolyse (MeSH), Lignine (composition chimique), Lignine (effets des radiations), Lignine (métabolisme), Lignine (ultrastructure), Microscopie électronique à balayage (MeSH), Populus (MeSH), Spectroscopie par résonance magnétique (MeSH), Température élevée (MeSH).
- MESH :
- composition chimique : Cellulose, Lignine.
- effets des radiations : Cellulose, Lignine.
- métabolisme : Cellulases, Cellulose, Lignine.
- ultrastructure : Cellulose, Lignine.
- Bois, Hydrolyse, Microscopie électronique à balayage, Populus, Spectroscopie par résonance magnétique, Température élevée.
English descriptors
- KwdEn :
- Cellulases (metabolism), Cellulose (chemistry), Cellulose (metabolism), Cellulose (radiation effects), Cellulose (ultrastructure), Hot Temperature (MeSH), Hydrolysis (MeSH), Lignin (chemistry), Lignin (metabolism), Lignin (radiation effects), Lignin (ultrastructure), Magnetic Resonance Spectroscopy (MeSH), Microscopy, Electron, Scanning (MeSH), Populus (MeSH), Wood (MeSH).
- MESH :
- chemical , chemistry : Cellulose, Lignin.
- chemical , metabolism : Cellulases, Cellulose, Lignin.
- chemical , radiation effects : Cellulose, Lignin.
- chemical , ultrastructure : Cellulose, Lignin.
- Hot Temperature, Hydrolysis, Magnetic Resonance Spectroscopy, Microscopy, Electron, Scanning, Populus, Wood.
Abstract
In dilute acid pretreatment of lignocellulosic biomass, lignin has been shown to form droplets that deposit on the cellulose surface and retard enzymatic digestion of cellulose (Donohoe et al., 2008; Selig et al., 2007). However, studies of this nature are limited for hydrothermal pretreatment, with the result that the corresponding mechanisms that inhibit cellulosic enzymes are not well understood. In this study, scanning electron microscope (SEM) and wet chemical analysis of solids formed by hydrothermal pretreatment of a mixture of Avicel cellulose and poplar wood showed that lignin droplets from poplar wood relocated onto the Avicel surface. In addition, nuclear magnetic resonance (NMR) showed higher S/G ratios in deposited lignin than the initial lignin in poplar wood. Furthermore, the lignin droplets deposited on Avicel significantly impeded cellulose hydrolysis. A series of tests confirmed that blockage of the cellulose surface by lignin droplets was the main cause of cellulase inhibition. The results give new insights into the fate of lignin in hydrothermal pretreatment and its effects on enzymatic hydrolysis.
DOI: 10.1002/bit.25108
PubMed: 24037461
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Investigation of lignin deposition on cellulose during hydrothermal pretreatment, its effect on cellulose hydrolysis, and underlying mechanisms.</title><author><name sortKey="Li, Hongjia" sort="Li, Hongjia" uniqKey="Li H" first="Hongjia" last="Li">Hongjia Li</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California, Riverside, California, 92507; Center for Environmental Research and Technology (CE-CERT), University of California, Riverside, California; BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California, Riverside, California, 92507; Center for Environmental Research and Technology (CE-CERT), University of California, Riverside, California; BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Pu, Yunqiao" sort="Pu, Yunqiao" uniqKey="Pu Y" first="Yunqiao" last="Pu">Yunqiao Pu</name></author><author><name sortKey="Kumar, Rajeev" sort="Kumar, Rajeev" uniqKey="Kumar R" first="Rajeev" last="Kumar">Rajeev Kumar</name></author><author><name sortKey="Ragauskas, Arthur J" sort="Ragauskas, Arthur J" uniqKey="Ragauskas A" first="Arthur J" last="Ragauskas">Arthur J. Ragauskas</name></author><author><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24037461</idno><idno type="pmid">24037461</idno><idno type="doi">10.1002/bit.25108</idno><idno type="wicri:Area/Main/Corpus">002463</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002463</idno><idno type="wicri:Area/Main/Curation">002463</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002463</idno><idno type="wicri:Area/Main/Exploration">002463</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Investigation of lignin deposition on cellulose during hydrothermal pretreatment, its effect on cellulose hydrolysis, and underlying mechanisms.</title><author><name sortKey="Li, Hongjia" sort="Li, Hongjia" uniqKey="Li H" first="Hongjia" last="Li">Hongjia Li</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California, Riverside, California, 92507; Center for Environmental Research and Technology (CE-CERT), University of California, Riverside, California; BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California, Riverside, California, 92507; Center for Environmental Research and Technology (CE-CERT), University of California, Riverside, California; BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Pu, Yunqiao" sort="Pu, Yunqiao" uniqKey="Pu Y" first="Yunqiao" last="Pu">Yunqiao Pu</name></author><author><name sortKey="Kumar, Rajeev" sort="Kumar, Rajeev" uniqKey="Kumar R" first="Rajeev" last="Kumar">Rajeev Kumar</name></author><author><name sortKey="Ragauskas, Arthur J" sort="Ragauskas, Arthur J" uniqKey="Ragauskas A" first="Arthur J" last="Ragauskas">Arthur J. Ragauskas</name></author><author><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name></author></analytic><series><title level="j">Biotechnology and bioengineering</title><idno type="eISSN">1097-0290</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cellulases (metabolism)</term><term>Cellulose (chemistry)</term><term>Cellulose (metabolism)</term><term>Cellulose (radiation effects)</term><term>Cellulose (ultrastructure)</term><term>Hot Temperature (MeSH)</term><term>Hydrolysis (MeSH)</term><term>Lignin (chemistry)</term><term>Lignin (metabolism)</term><term>Lignin (radiation effects)</term><term>Lignin (ultrastructure)</term><term>Magnetic Resonance Spectroscopy (MeSH)</term><term>Microscopy, Electron, Scanning (MeSH)</term><term>Populus (MeSH)</term><term>Wood (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Bois (MeSH)</term><term>Cellulases (métabolisme)</term><term>Cellulose (composition chimique)</term><term>Cellulose (effets des radiations)</term><term>Cellulose (métabolisme)</term><term>Cellulose (ultrastructure)</term><term>Hydrolyse (MeSH)</term><term>Lignine (composition chimique)</term><term>Lignine (effets des radiations)</term><term>Lignine (métabolisme)</term><term>Lignine (ultrastructure)</term><term>Microscopie électronique à balayage (MeSH)</term><term>Populus (MeSH)</term><term>Spectroscopie par résonance magnétique (MeSH)</term><term>Température élevée (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cellulose</term><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulases</term><term>Cellulose</term><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="radiation effects" xml:lang="en"><term>Cellulose</term><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="ultrastructure" xml:lang="en"><term>Cellulose</term><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Cellulose</term><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="effets des radiations" xml:lang="fr"><term>Cellulose</term><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellulases</term><term>Cellulose</term><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Cellulose</term><term>Lignine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Hot Temperature</term><term>Hydrolysis</term><term>Magnetic Resonance Spectroscopy</term><term>Microscopy, Electron, Scanning</term><term>Populus</term><term>Wood</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Bois</term><term>Hydrolyse</term><term>Microscopie électronique à balayage</term><term>Populus</term><term>Spectroscopie par résonance magnétique</term><term>Température élevée</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In dilute acid pretreatment of lignocellulosic biomass, lignin has been shown to form droplets that deposit on the cellulose surface and retard enzymatic digestion of cellulose (Donohoe et al., 2008; Selig et al., 2007). However, studies of this nature are limited for hydrothermal pretreatment, with the result that the corresponding mechanisms that inhibit cellulosic enzymes are not well understood. In this study, scanning electron microscope (SEM) and wet chemical analysis of solids formed by hydrothermal pretreatment of a mixture of Avicel cellulose and poplar wood showed that lignin droplets from poplar wood relocated onto the Avicel surface. In addition, nuclear magnetic resonance (NMR) showed higher S/G ratios in deposited lignin than the initial lignin in poplar wood. Furthermore, the lignin droplets deposited on Avicel significantly impeded cellulose hydrolysis. A series of tests confirmed that blockage of the cellulose surface by lignin droplets was the main cause of cellulase inhibition. The results give new insights into the fate of lignin in hydrothermal pretreatment and its effects on enzymatic hydrolysis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24037461</PMID><DateCompleted><Year>2014</Year><Month>09</Month><Day>15</Day></DateCompleted><DateRevised><Year>2014</Year><Month>01</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1097-0290</ISSN><JournalIssue CitedMedium="Internet"><Volume>111</Volume><Issue>3</Issue><PubDate><Year>2014</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Biotechnology and bioengineering</Title><ISOAbbreviation>Biotechnol Bioeng</ISOAbbreviation></Journal><ArticleTitle>Investigation of lignin deposition on cellulose during hydrothermal pretreatment, its effect on cellulose hydrolysis, and underlying mechanisms.</ArticleTitle><Pagination><MedlinePgn>485-92</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/bit.25108</ELocationID><Abstract><AbstractText>In dilute acid pretreatment of lignocellulosic biomass, lignin has been shown to form droplets that deposit on the cellulose surface and retard enzymatic digestion of cellulose (Donohoe et al., 2008; Selig et al., 2007). However, studies of this nature are limited for hydrothermal pretreatment, with the result that the corresponding mechanisms that inhibit cellulosic enzymes are not well understood. In this study, scanning electron microscope (SEM) and wet chemical analysis of solids formed by hydrothermal pretreatment of a mixture of Avicel cellulose and poplar wood showed that lignin droplets from poplar wood relocated onto the Avicel surface. In addition, nuclear magnetic resonance (NMR) showed higher S/G ratios in deposited lignin than the initial lignin in poplar wood. Furthermore, the lignin droplets deposited on Avicel significantly impeded cellulose hydrolysis. A series of tests confirmed that blockage of the cellulose surface by lignin droplets was the main cause of cellulase inhibition. The results give new insights into the fate of lignin in hydrothermal pretreatment and its effects on enzymatic hydrolysis.</AbstractText><CopyrightInformation>© 2013 Wiley Periodicals, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Hongjia</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California, Riverside, California, 92507; Center for Environmental Research and Technology (CE-CERT), University of California, Riverside, California; BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pu</LastName><ForeName>Yunqiao</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Kumar</LastName><ForeName>Rajeev</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Ragauskas</LastName><ForeName>Arthur J</ForeName><Initials>AJ</Initials></Author><Author ValidYN="Y"><LastName>Wyman</LastName><ForeName>Charles E</ForeName><Initials>CE</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>10</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biotechnol Bioeng</MedlineTA><NlmUniqueID>7502021</NlmUniqueID><ISSNLinking>0006-3592</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="D044602">Cellulases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D044602" MajorTopicYN="N">Cellulases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000528" MajorTopicYN="N">radiation effects</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006358" MajorTopicYN="N">Hot Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000528" MajorTopicYN="N">radiation effects</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009682" MajorTopicYN="N">Magnetic Resonance Spectroscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008855" MajorTopicYN="N">Microscopy, Electron, Scanning</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">deposition</Keyword><Keyword MajorTopicYN="N">enzymatic hydrolysis</Keyword><Keyword MajorTopicYN="N">hydrothermal pretreatment</Keyword><Keyword MajorTopicYN="N">inhibition mechanisms</Keyword><Keyword MajorTopicYN="N">lignin droplets</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>06</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>08</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>08</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>9</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>9</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>9</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24037461</ArticleId><ArticleId IdType="doi">10.1002/bit.25108</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Tennessee</li></region></list><tree><noCountry><name sortKey="Kumar, Rajeev" sort="Kumar, Rajeev" uniqKey="Kumar R" first="Rajeev" last="Kumar">Rajeev Kumar</name><name sortKey="Pu, Yunqiao" sort="Pu, Yunqiao" uniqKey="Pu Y" first="Yunqiao" last="Pu">Yunqiao Pu</name><name sortKey="Ragauskas, Arthur J" sort="Ragauskas, Arthur J" uniqKey="Ragauskas A" first="Arthur J" last="Ragauskas">Arthur J. Ragauskas</name><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name></noCountry><country name="États-Unis"><region name="Tennessee"><name sortKey="Li, Hongjia" sort="Li, Hongjia" uniqKey="Li H" first="Hongjia" last="Li">Hongjia Li</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002156 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002156 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:24037461
|texte= Investigation of lignin deposition on cellulose during hydrothermal pretreatment, its effect on cellulose hydrolysis, and underlying mechanisms.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24037461" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |